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Circulating mesenchymal stem cells with abnormal osteogenic differentiation in patients with osteoporosis
Arthritis & Rheumatism, 11/09/09

Carbonare LD et al. – These results suggest that an alteration of osteoblastic differentiation may contribute to the pathogenesis of osteoporosis. The noninvasive approach used in the present study could be proposed as a useful tool for studying mesenchymal involvement in bone diseases.

Methods

Osteogenic differentiation process in MSCs obtained from peripheral blood of 31 patients with osteoporosis and 20 normal donors
Cells evaluated by colony-forming unit-fibroblastic assay and cultured in osteogenic medium to analyze transcription factors runt-related transcription factor 2 (RUNX-2) and Sp7 and bone-related genes COL1A1, SPARC, and SPP1 after 3, 8, and 15 days of differentiation
To determine possible differences between 2 groups in terms of osteoclastic and osteoblastic activation, we quantified osteoprotegerin (OPG) and RANKL levels in supernatants of osteoblastic culture

Results

Circulating MSCs increased in osteoporosis patients compared with normal donors
In contrast, gene expression analysis revealed down-regulation of RUNX2, Sp7, COL1A1, SPARC, and SPP1 in patients with osteoporosis, associated with lower OPG:RANKL ratio

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