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Comparative study on circulating endothelial progenitor cells in systemic lupus erythematosus patients at active stage
Rheumatology International, 10/28/09

Deng XL et al. – Circulating endothelial progenitor cells (CEPCs) play an important role in the process of atherosclerosis.The purpose of this study was to determine the number, function , and the integrated status of CEPCs in active SLE patients. Data from this study show that CEPC number in active SLE patients was not significantly different from healthy controls, but their functions were partly impaired, including proliferation, adhesion, migration, and tube formation. Bad cell status and increased susceptibility to inflammatory process of CEPCs in active SLE were also observed in our study.

Methods

35 active SLE patients (28 females, 7 males) and 35 age-and gender-matched healthy controls
CEPC number determined by Fluorescence-Activated Cell Sorting
Proliferation capacity of CEPC assessed by PCNA staining
Adhesion capacity of CEPC to fibronectin and adhesion capacity of THP1 cell to CEPC were determined by cell adhesion assay
Migratory capacity of CEPC measured by transwell chamber assay and the potential to form tubes on Matrigel of CEPC was determined by in vivo tube formation on Matrigel test
Expression of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) assessed by quantitative PCR as well as expression of intercellular adhesion molecule-1 (ICAM-1) and phosphorylated-Akt (p-Akt) assessed by western-blotting used to evaluate the anti-inflammatory function and cell status of CEPCs

Results

Number of CEPC in SLE patients not different from that in control
Proliferation capacity of CEPC decreased in active SLE patients
Adhesion capacity of CEPC to fibronectin decreased in SLE patients and adhesion capacity of THP1 cell to CEPC increased in SLE patients
Migratory activity reduced in patient CEPCs
Capacity of CEPCs to form tube on Matrigel was decreased in SLE patients
Expression of iNOS and IL-6 and ICAM-1 increased in CEPC of SLE patients and expression of p-Akt was decreased in CEPC of SLE patients

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