Rheumatology Medical News about juvenile idiopathic arthritis;Th17 transcription factor RORC2;FOXP3 expression

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Th17 transcription factor rorc2 is inversely correlated with FOXP3 expression in the joints of children with juvenile idiopathic arthritis
Journal of Rheumatology, 09/09/09

Olivito B et al. – There is an increased synovial expression of the transcription factor of Th17, RORC2, in JIA, and its inverse relationship with FOXP3 mRNA.

Methods

65 children with JIA (18 males and 47 females; median age, 6.2 yrs; 45 with oligoarticular and 20 with polyarticular ds) and 75 age- and gender-matched healthy controls were studied
Flow cytometry was used to analyze the forkhead box P3 (FOXP3)-positive Treg cells in peripheral blood and synovial fluid mononuclear cells
FOXP3 and retinoic-acid related orphan receptor C isoform 2 (RORC2) mRNA were assessed by real-time polymerase chain reaction analysis
Cytokines (IL-17 and Th1/Th2 related cytokines) were measured in culture supernatants of 11 paired PBMC and SFMC activated with PMA and ionomycin

Results

FOXP3+ T cells and FOXP3 mRNA amounts were significantly lower in PB of children with JIA as compared with controls and a higher percentage of Treg cells with concomitant higher level of FOXP3 transcript levels were observed in SF when compared with their PB counterparts
SF CD4+FOXP3+ T cells were characterized by higher amounts of FOXP3 protein per cell when compared with peripheral CD4+FOXP3+ T cells, as revealed by the difference in FOXP3 median fluorescence intensity (median ± SD, arbitrary units, 54 ± 22.6 vs 19.5 ± 4.2)
RORC2 transcript levels were higher in JIA joints when compared with matched PB samples (median fold increase 3.9) but negatively correlated with FOXP3 mRNA levels (r = ?0.623)
Stimulated SFMC displayed an impaired ability to produce IL-17 when compared with PBMC and, interestingly, an inverse relationship between IL-17 levels and the percentage of CD4+CD25+FOXP3+ SF T cells (r = ?0.510) was seen

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