Rheumatology Medical News about Proteomics, SIPS, SELDI-TOF-MS

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Proteomics analysis in 28 patients with systemic IgG4-related plasmacytic syndrome
Rheumatology International, 07/24/09

Yamamoto M et al. – Study reports that proteomic analysis revealed a specific protein peak of 13.1 kDa in samples from all patients with Mikulicz’s disease (MD) and autoimmune pancreatitis (AIP) patients, but never in those from healthy controls (HC) and Sjögren’s syndrome (SS). There were other peaks in SIPS patients, but they were suggested to be individual differences.

Methods

Systemic IgG4-related plasmacytic syndrome (SIPS) is comprised of: AIP, MD and IgG4-related tubulointerstitial nephritis (TIN)
Study analyzes the serological aspects of SIPS to investigate whether there are autoantigens in SIPS
28 pts with SIPS; mainly middle-aged (8 male), consisted of 26 pts with MD and 2 with AIP
3/26 pts diagnosed with MD were complicated to AIP, and another 3 pts had IgG4-related TIN
As a control, healthy volunteers and the pts with SS were examined
Levels of serum complements and circulating immune complexes in these pts were measured
Next, immune complexes were collected from the serum of pts and healthy controls by immunoprecipitation
They were divided into immunoglobulin and the antigens by glycine–HCl solution
The divided samples including the antigens were analyzed by SELDI-TOF-MS

Results

9 pts had hypocomplementemia and 15 had elevated levels of circulating immune complexes in the group of SIPS
Healthy volunteers and SS pts showed normal levels of serum complements and circulating immune complexes
SELDI-TOF-MS detected a 13.1-kDa protein from all samples of SIPS, and not in normal control and SS
It is possible that the 13.1-kDa protein is one of the autoantigens of SIPS

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