Rheumatology Medical News about human articular cartilage;degradome expression profiling

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Degradome expression profiling in human articular cartilage
Arthritis Research & Therapy, 06/25/09

Swingler TE et al. – This study provides a complete quantitative analysis of protease gene expression in cartilage; gives direction to future research on the specific function(s) of individual proteases or protease families in cartilage and may help to refine anti-proteolytic strategies in osteoarthritis (OA).

Methods

Expression profile of protease genes in all catalytic classes in normal human cartilage and from OA pts
Human cartilage obtained from femoral heads at joint replacement for either OA or following fracture to the neck of femur (NOF)
Total RNA was purified, and expression of genes assayed using Taqman(R) low density array qRT-PCR

Results

538 protease genes were profiled, of which 431 were expressed in cartilage
179 genes were differentially expressed in OA vs NOF cartilage: 8 aspartic proteases, 44 cysteine proteases, 76 metalloproteases, 46 serine proteases and 5 threonine proteases
Wilcoxon ranking as well as the LogitBoost-NR machine learning approach were used to assign significance to each gene, with the most highly ranked genes broadly similar using each method

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