Rheumatology Medical News about synovial fluid level of aggrecan ARGS fragments;narker of joint disease;glycosaminoglycan;aggrecan

Become a Member Today!
Register

Most Viewed Abstracts

1. Report Shows Shift in Starting Salaries for Physicians

2. Rapid correction of low vitamin D status in nursing home residents

3. 2008 Exclusive Survey—Earnings: Good news for primary care income

4. Medicare pay-for-reporting effort draws fire from frustrated doctors

5. Allopurinol-induced recurrent dress syndrome: Pathophysiology and treatment

Your Article Summary

See Latest Articles

(Click the title below to leave the MDLinx Network and go to the Journal's Website)

Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: A cross-sectional study
Arthritis Research & Therapy, 06/24/09

Larrson S et al. – In this cross-sectional study, comparing knee of healthy persons with acute inflammatory arthritis, acute knee injury, chronic knee injury and knee osteoarthritis, the SF levels of the aggrecan ARGS neoepitope were quantified with a modified sandwich ELISA and was compared with aggrecan assays not specific for this neoepitope. Data show that ARGS neoepitope concns in SF differ between these groups and this method is more sensitive measure of joint disease than previously used aggrecan or sGAG assays.

Methods

A quantitative immunoassay of aggrecan ARGS neoepitope fragments in human SF to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays
SF from 26 knee healthy persons and 269 pts were analyzed
Patient groups: acute inflammatory arthritis, acute knee injury, chronic knee injury and knee OA
Aggrecan ARGS fragments were assayed by ELISA using the monoclonal antibody OA-1
Total aggrecan content was analyzed by an ELISA using the monoclonal antibody 1-F21, and sulfated glycosaminoglycan by Alcian blue precipitation

Results

Aggrecan ARGS fragment concns in all groups differed from the reference group
Acute inflammatory arthritis group had the highest median level; >177-fold vs reference group
Median levels were: reference 0.5, acute inflammatory arthritis 88.5, acute knee injury 53.9, chronic knee injury 0.5 and OA 4.6
In contrast, aggrecan and sulfated glycosaminoglycan concns varied much less between groups
Only acute inflammatory arthritis and acute knee injury showed 2-fold increase in median levels vs reference

<< Previous
Antiphospholipid antibody profiles in lupus nephritis with...

Next >>
Prognostic factors for lung function in systemic sclerosis:...