Baldini C et al. - A salivary 12 kDa psoriasin (S100A7) as a potential predictor of pulmonary involvement in SSc was identified. Thus, a psoriasin assay might be considered as a rapid, noninvasive, useful salivary biomarker for the detection of pulmonary involvement in SSc. Methods
Study to evaluate psoriasin expression in whole saliva (WS) of pts with diffuse systemic sclerosis (dSSc) and limited SSc (lSSc), and to correlate its presence with the different clinical manifestations
44 pts with limited or diffuse SSc were enrolled for study
WS proteins were separated by SDS gel electrophoresis and psoriasin was identified by Western blot analysis
Pts with other rheumatic diseases with and w/o lung involvement were enrolled as pathological controls
Results
3 bands of 12, 24, and 50 kDa corresponding to monomeric and dimeric/multimeric forms of psoriasin were evidenced by immunoblot analysis in WS of 31 out of 44 patients with SSc (70.4%)
In the other 13 WS samples, the 12 kDa band was totally absent, while the dimeric and multimeric bands were expressed at optical intensity (OD) levels comparable to the other samples
Presence of 12 kDa monomeric psoriasin was associated with SSc pulmonary involvement and with anti-Scl-70 antibody positivity
See all
