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Fenollar F et al. – Broad-range PCR followed by sequencing offers several advantages when used to complement culture results for the diagnosis of fastidious bacteria and for patients taking antibiotics. However, its use should be restricted mainly for culture-negative cases when infection is suspected on the basis of clinical signs and symptoms or inflammatory syndrome. Methods- A review to describe the general principles and the usefulness of broad-range PCR to improve the etiological diagnosis of osteoarticular infections
Results- Broad-range PCR followed by sequencing has been successfully developed to identify microorganisms involved in infections when pts have previously received antibiotics or in the presence of slow-growing or intracellular microorganisms
- For osteoarticular infections, the studies have shown that the use of this molecular tool increased mainly the identification of Kingella kingae, anaerobic bacteria, and Streptococcus sp
- However, it is very important to underline that the interpretation of this molecular tool is critical because of several pitfalls, including contamination causing false-positive results
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