Mansour RB et al. – The primary factor causing the oxidative stress observed in RA and SLE is excessive free radical production rather than impaired catalase (CAT) or superoxide dismutase (SOD) activity due to autoantibody inhibition. Methods
Study t o evaluate the level of autoantibodies against SOD and CAT in the sera of RA and SLE Tunisian pts and their role in the genesis of the two diseases
Using a standard ELISA, the levels of IgG and IgM directed against CAT and SOD in the sera of 39 RA pts, 40 SLE pts, and 50 control healthy individuals were evaluated
The oxidative/antioxidative profile was tested by measuring serum malondialdehyde (MDA), conjugated dienes (CD), CAT activity, and SOD activity
Results
Increased levels of IgG antibodies against CAT in both groups of pts vs control subjects was observed
However, the SLE pts displayed an increased level of anti-SOD IgG
In all pts the lipid peroxidation was confirmed by high levels of MDA and conjugated dienes
RA pts exhibited an increasing CAT and SOD activity in their sera with a positive correlation observed between CAT and IgG anti-CAT
The same results were observed for SLE pts
In addition, a positive correlation was observed between anti-CAT Ab and anti-SOD Ab in SLE pts
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