C. Poulopoulou C et al. – An enhancing in vivo effect of low serum glutamate concentrations on the functional activity of Kv1.3 channel may contribute to lupus T cell hyperactivity. Methods
A study to examine serum glutamate concentrations and Kv1.3 channel activity in SLE pts
HPLC was used for glutamate measurements
Whole-cell patch-clamp technique for electrophysiologic studies was performed in freshly isolated, noncultured peripheral T cells
Results
Mean serum concs of glutamate were lower in pts with either clinically quiescent SLE or active SLE than in healthy controls
The intrinsic gating properties of the Kv1.3 channels in lupus T cells were comparable to healthy control-derived T cells
Extracellular glutamate concs similar to their respective serum levels demonstrated Kv1.3 current responses enhanced by almost 20% vs glutamate concs within control serum levels
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