Gosset M et al. – The findings of this study indicate that visfatin, an adipose tissue-derived hormone has a catabolic function in cartilage and may have an important role in the pathophysiology of OA. Methods
A study to investigate the contribution of visfatin to the pathophysiology of OA, by examining its role in PGE2 synthesis and matrix degradation
The synthesis of visfatin with and w/o stimulation with IL-1β and the role of visfatin in PGE2 synthesis were analyzed by RT-PCR and immunoblotting
The effects of visfatin on mPGES-1 and 15-PGDH synthesis, on the subsequent release of PGE2, and on MMP-3, MMP-13, ADAMTS-4, ADAMTS-5, and PG synthesis were examined by qRT-PCR, immunoblotting, and ELISA
siRNA was used to assess the influence of visfatin on IL-1β-induced release of PGE2 in immature mouse articular chondrocytes
Results
Human OA chondrocytes produced visfatin, and visfatin synthesis was increased by IL-1β treatment
Visfatin, like IL-1β, triggered excessive release of PGE2, due to increased mPGES-1 synthesis and decreased 15-PGDH synthesis
Visfatin knockout with siRNA reduced IL-1β-induced PGE2 overrelease
Visfatin triggered ADAMTS-4 and ADAMTS-5 expression and MMP-3 and MMP-13 synthesis and release
It reduced synthesis of high molecular weight PG by immature mouse articular chondrocytes
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