Wildenberg ME et al. – Mature monocytes are increased in primary Sjögren’s Syndrome (pSjS) and both patient and mouse data support a model where this mature monocyte subset migrates to the salivary glands and develops into dendritic cells (DC). Methods
Aim was to determine the level of mature CD14llowCD16+ monocytes in pSjS and their contribution to the accumulation of DC in pSjS
Levels of mature and immature monocytes in pSjS pts (n=19) and controls (n=15) were analyzed by flow cytometry
The reverse transmigration system was used for generation of DC generated from monocyte subsets
The phenotype of DC in pSjS salivary glands was analyzed using IHC
In vivo tracking of monocyte subsets was performed in a mouse model
Results
Increased levels of mature CD14lowCD16+ monocytes were found in pSjS pts
These cells showed a normal expression of chemokine receptor and adhesion molecules
Mature monocytes partly developed into DC-LAMP+ and CD83+ DC, markers also expressed by DC in pSjS salivary glands
Homologue population of mature mouse monocytes migrated to the salivary glands, and preferentially developed into CD11c+ DC in vivo
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