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Connective tissue growth factor, a regulator related with 10-hydroxy-2-decenoic acid down-regulate MMPs in rheumatoid arthritis
Rheumatology International, 08/23/2011
Wang JG et al. – The results suggest that connective tissue growth factor (CTGF) is a regulator factor in the expression of matrix metalloproteinases (MMPs), and 10H2DA down–regulate the concentration of MMPs probably by down–regulating the expression of CTGF.
Methods- Two–dimensional electrophoresis (2–DE) and LC–MS/MS were performed to identify changes in protein expression after 24–h 10H2DA treatment.
- Differentially expressed proteins were identified by real–time PCR and Western blot analysis.
- Influence of down–regulation of connective tissue growth factor (CTGF) expression on MMPs was studied by RNAi.
- Ten proteins were up–regulated and 9 proteins were down–regulated after 24–h 10H2DA treatment.
- A total of 19 differentially expressed proteins were identified and found to be associated with glycolysis, lipid metabolism, cell adhesion, ATP synthesis, oxidation reduction, and anti–apoptosis.
- CTGF, a member of the C–terminal cystein–rich proteins (CCN) family, was down–regulated after 24–h 10H2DA treatment.
- MMPs were down–regulated after RNAi (CTGFi).
