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Serum IgA against type 3 muscarinic acetylcholine receptor is a novel marker in diagnosis of Sjogren’s syndrome Full Text
Chinese Medical Journal, 08/29/2011
Ying-ni LI et al. – The improved anti-c2M3RP ELISA is a novel, sensitive, and specific serological test for the diagnosis of SS. The combined application of anti-c2M3RP, anti-SSA and anti-SSB tests can improve the laboratory diagnosis of SS. The IgA anti-c2M3RP antibodies may serve as a novel diagnostic marker for SS.
Methods- To increase ELISA sensitivity, c2M3RP was coupled to bovine serum albumin (BSA) by the glutaraldehyde method and a 96-well microplate was treated by ultraviolet rays before coated
- Concentrations of anti-c2M3RP, anti-SSA, and anti-SSB were measured in the sera of 240 individuals: 91 patients with primary SS and 149 controls (16 secondary SS, 27 SLE, 40 RA and 66 healthy controls). Diagnostic properties of anti-c2M3RP determined by receiver-operating characteristic curve analysis
- Prevalence of serum IgA anti-c2M3RP antibodies in patients with pSS (46%, 42/91) was significantly higher than that in patients with SLE (19%, 5/27), in RA (15%, 6/40) and in healthy controls (5%, 3/66)
- No significant difference between 2 SS groups (P=0.727)
- Diagnostic performance of IgA anti-M3RP antibodies was similar to anti-SSA assay, but had 22% higher sensitivity than anti-SSB
- By analyzing of IgA anti-c2M3RP antibodies, combination of anti-SSA and anti-SSB resulted in increased sensitivity, whereas their specificity was not significantly changed
