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Analysis of granulysin-mediated cytotoxicity in peripheral blood of patients with psoriatic arthritis
Rheumatology International, 08/16/2011
Massari D et al. – The findings demonstrated the involvement of granulysin (GNLY) in the worsening of psoriatic arthritis (PsA) and suggested that GNLY mediated the development of joint lesions.
Methods- The authors prospectively enrolled 25 PsA patients in the active phase, 26 PsA patients in remission and 24 healthy controls.
- The simultaneous detection of intracellular GNLY and cell surface antigens (CD3 and CD56) was performed with flow cytometry.
- GNLY apoptotic protein was visualised by immunocytochemistry.
- Natural killer (NK) cell cytotoxicity was analysed with a cytotoxicity assay against human erythroleukaemia K–562 cells.
- The percentage of GNLY+ cells did not differ significantly between PsA patients in the acute phase and those in remission; however, it was always higher than in healthy examinees due to the increased percentage of GNLY+ cells within T cells, NKT cells, and both, and in the CD56+dim and CD56+bright NK subsets.
- The mean fluorescence intensity for GNLY was higher in all lymphocyte subpopulations in the acute phase than in remission and in healthy controls.
- Accordingly, GNLY–mediated NK cell cytotoxicity against K–562 cells of active phase PsA patients was significantly higher than that in patients in remission or in healthy controls.
