Disruption of rhythms of molecular clocks in primary synovial fibroblasts of patients with osteoarthritis and rheumatoid arthritis, role of interleukin1 beta and tumor necrosis factor Full Text
Arthritis Research & Therapy,

Haas S et al. – Rhythmicity is not present in primary OASF and RASF, which is unexpected because fibroblasts usually demonstrate perfect rhythms during several days. This might lead to uncoupling of important cellular pathways.

Methods
  • Presence of BMAL–1, CLOCK, Period 1, and Period 2 proteins in synovial tissue was investigated by immunofluorescence.
  • Presence of mRNA of molecular clocks was studied during 72hr by qPCR.
  • Characteristics of rhythms were studied with time series analysis.

Results
  • BMAL–1, CLOCK, Period 1, and Period 2 proteins were abundantly present in synovial tissue of osteoarthritis (OA), rheumatoid arthritis (RA), and controls.
  • Receiving synovial tissue at different operation time points during the day (8.00am–4.00pm) did not reveal a rhythm of BMAL–1 or Period 1 protein.
  • In OASF and RASF, no typical rhythm curve of molecular clock mRNA was observed.
  • Time series analysis identified a first peak between 2 and 18 hours after synchronization but a period was not detectable due to loss of rhythm.
  • TNF inhibited mRNA of CLOCK, Period 1, and Period 2 in OASF, while IL–1beta and TNF increased these factors in RASF.
  • This was supported by dose–dependently increased levels in MH7A RA fibroblasts.
  • In RASF, IL 1beta and TNF shifted the first peak of BMAL–1 mRNA to later time points (8hr to 14hr).

Please login or register to follow this author.
Are you sure you want to Unfollow this Author?
► Click here to access Full Text, PubMed, Publisher and related articles...
<< Previous Article | Next Article >>

Your Unread Messages in Rheumatology

See All >> Messages include industry-sponsored communications and special communications from MDLinx

Most Popular Rheumatology Articles

1 Homeopathy in the treatment of fibromyalgia- A comprehensive literature-review and meta-analysis Complementary Therapies in Medicine, August 15, 2014    Evidence Based Medicine    Review Article

2 Lupus classification criteria expanded; treat-to-target approach gains support Full Text The Rheumatologist, July 24, 2014    Free full text

3 Rituximab for the treatment of relapses in ANCA-associated vasculitis Arthritis & Rheumatism, August 1, 2014    Clinical Article

4 Oral bisphosphonate use and total knee/hip implant survival: Validation of results in an external population-based cohort Arthritis & Rheumatism, August 14, 2014    Clinical Article

5 Gout, urate lowering therapy and uric acid levels among US adults Arthritis Care & Research , September 16, 2014    Clinical Article

6 Frequent conversion of tuberculosis screening tests during anti-tumour necrosis factor therapy in patients with rheumatic diseases Annals of Rheumatic Diseases, September 10, 2014    Clinical Article

7 Does non-erosive rheumatoid arthritis exist? a cross-sectional analysis and a systematic literature review Seminars in Arthritis and Rheumatism, September 25, 2014    Review Article

8 Valgus bracing for knee osteoarthritis: A meta-analysis of randomized trials Arthritis Care & Research , September 12, 2014    Evidence Based Medicine

9 Early predictors of prognosis in juvenile idiopathic arthritis: A systematic literature review Annals of Rheumatic Diseases, August 29, 2014    Review Article

10 Potential risk of TNF inhibitors on the progression of interstitial lung disease in patients with rheumatoid arthritis BMJ Open, September 12, 2014    Clinical Article

11 Immunosuppressive therapies for the induction treatment of proliferative lupus nephritis: A systematic review and network metaanalysis The Journal of Rheumatology, September 18, 2014    Evidence Based Medicine    Review Article

12 Adalimumab in patients with hand osteoarthritis refractory to analgesics and NSAIDs: A randomised, multicentre, double-blind, placebo-controlled trial Annals of Rheumatic Diseases, August 25, 2014    Clinical Article

13 Profound reduction in hospital admissions and musculoskeletal surgical procedures for rheumatoid arthritis with concurrent changes in clinical practice (1995-2010) Rheumatology, September 26, 2014    Clinical Article

14 Tocilizumab in rheumatoid arthritis: A case study of safety evaluations of a large postmarketing data set from multiple data sources Seminars in Arthritis and Rheumatism, September 29, 2014    Clinical Article

15 Differentiation between osteoarthritis and psoriatic arthritis: Implications for pathogenesis and treatment in the biological therapy era Rheumatology, September 22, 2014    Review Article

16 Pain management with acupuncture in osteoarthritis: a systematic review and meta-analysis Full Text BMC Complementary and Alternative Medicine, September 8, 2014    Free full text    Evidence Based Medicine    Review Article

17 Drug-associated cutaneous vasculitis: Study of 239 patients from a single referral center The Journal of Rheumatology, September 25, 2014    Clinical Article

18 “Worm pill” could ease autoimmune disease symptoms Monash University News, August 12, 2014

19 Comparison of the efficacy and safety of two starting dosages of prednisolone in early active rheumatoid arthritis (CORRA): study protocol for a randomized controlled trial Full Text Trials, September 18, 2014    Free full text    Evidence Based Medicine    Clinical Article

20 A possible link between ankylosing spondylitis and periodontitis: A systematic review and meta-analysis Rheumatology, September 16, 2014    Evidence Based Medicine    Review Article

Indexed Journals in Rheumatology: Arthritis & Rheumatism, Annals of Rheumatic Diseases, Journal of Rheumatologymore

Register now to view all the MDLinx contents (FREE)!

  • Stay current on the latest literature, research and clinical news
  • Get special communications and offers from MDLinx and our sponsors
  • Receive invitations to paid market research
View Samples and Register

Connect with us, stay current.

Receive the latest mecial news
updates for free via email

Sign up!

Subscribe to our free RSS feeds:

Get the latest news in your specialty automatically added to your newsreader or your personal My Yahoo!, Google, My MSN or My AOL page. Learn More

Close