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Zheng S et al. - The cryoprotective abilities of dimethyl sulfoxide as a function of its concentration in saline and storage temperature were also investigated. A 30% DMSO concentration was sufficient in preventing the reduction of GAG in the tissue at the -20°C storage temperature, but a 50% concentration of DMSO was necessary for the -80°C cryopreservation. These imaging results were verified by the biochemical analysis.


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