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Happonen, A.P., et al. - Single photon emission computed tomography (SPECT) provides a method to obtain three-dimensional images of blood perfusion. In cardiac stress–rest SPECT studies, coronary artery disease can be examined by comparing intensity differences of the acquired images of the heart at stress and rest. The stress–rest image pair needs to be aligned into the same spatial position and orientation in order to compare the images in an automated and reproducible manner

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