Tissue micro array analysis of ganglioside N-glycolyl GM3 expression and signal transducer and activator of transcription (STAT)-3 activation in relation to dendritic cell infiltration and microvessel density in non-small cell lung cancer
van Cruijsen H et al. - In a study to assess the possible association of ganglioside GM3 expression and signal transducer and activator of transcription (STAT)-3 activation with suppression of dendritic cell (DC) activation and angiogenesis in non-small cell lung cancer (NSCLC), it was shown that N-glycolyl GM3 (GM3) and phosphorylated STAT3 (pSTAT3) are widely expressed in NSCLC. Based on CD83 expression, GM3, but not pSTAT3, appeared to be involved in tumor-induced DC suppression. pSTAT3 expression was not associated with microvessel density (MVD), while GM3 might play an anti-angiogenic role. Methods- Immunohistochemistry was performed on a tissue array to determine GM3 and pSTAT3 expression in 176 primary NSCLC resections.
- Median values of GM3 and pSTAT3 expression were used as cut off.
- MVD was determined by CD34 staining and morphology.
- CD1a and CD83 were used to determine infiltrating immature and mature dendritic cells, respectively.
Results- 94% and 71% of the NSCLC samples expressed GM3 and nuclear pSTAT3, respectively.
- Median overall survival was 40.0 mos.
- Both low GM3 expression and high pSTAT3 expression were associated with worse survival, which reached near significance for GM3.
- MVD, determined by CD34 staining and morphology, was lower in NSCLC samples with high GM3 expression.
- CD1a+ cells (immature DCs) were more frequent in NSCLC tissues vs peritumoral lung tissue, while CD83+ cells (mature DCs) were more frequent in peritumoral lung tissue.
- CD83+ DCs were less frequent in NSCLC tissues with high GM3 expression.
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