A prospective PCR-based screening for the EML4-ALK oncogene in non-small cell lung cancer
Clinical Cancer Research, 08/21/2012
Soda M et al. – Multiplex reverse transcription (RT)–PCR was confirmed to be a reliable technique for detection of ALK fusion transcripts. The authors propose that diagnostic tools for EML4–ALK should be selected in a manner dependent on the available specimen types. FISH and sensitive immunohistochemistry should be applied to formalin–fixed, paraffin–embedded tissue, but multiplex RT–PCR is appropriate for other specimen types.