Low 2-methoxyestradiol levels at the first trimester of pregnancy are associated with the development of pre-eclampsia
Prenatal Diagnosis, 08/09/2012
Perez–Sepulveda A et al. – Lower plasma concentrations of 2–ME during early pregnancy in patients who subsequently develop pre–eclampsia (PE) were found. Presence of placental Val158Met COMT polymorphism is associated with a decreased risk to develop PE, suggesting a protective role against PE.Methods
- Clinical characteristics and plasma samples were collected at 11 to 14 weeks prospectively in a cohort of patients.
- From them, 13 PE and 72 control pregnant women were chosen.
- Plasma soluble fms–like tyrosine kinase1 and placental growth factor levels were measured by electrochemiluminescence and 2–ME was measured by high–performance liquid chromatography with mass spectrometry/mass spectrometry detection.
- At delivery, placental tissue was collected and the Val158Met COMT polymorphism was determined by restriction fragment length polymorphism–PCR.
- At 11 to 14 weeks, patients who would develop PE have significantly lower plasma levels of 2–ME than controls [1.9±2 standard error of the mean (SEM) vs 61.7±27 pg/mL, P<0.05].
- The Val158 Met polymorphism was more frequent in controls than in PE patients and the placental presence of COMT polymorphism was associated with a decreased risk of developing PE [PE: 23.1% vs control: 66.6%; 2=10.9, p=0.0041].