HLA-DRβ1*04 typing by simple in-house PCR-SSP technique for rheumatoid arthritis patients
Rheumatology International, 06/15/2012
Parasannanavar DJ et al. – In this study, a very simple, convenient and more cost–effective in–house polymerase chain reaction (PCR)–SSP technique was standardized for human leukocyte antigen (HLA)–DR Β1*04 typing that is helpful to rheumatoid arthritis (RA) diagnosis in developing countries like India, which can be used as a good screening test.
- A strong association between rheumatoid arthritis (RA) and human leukocyte antigen (HLA) has been observed in many different populations and that accounts for approximately one–third of the genetic component of RA susceptibility.
- The greatest effect comes from DR β1 gene where the strongest association has been found with DR β1*04 (DR4) allele.
- As serology has some disadvantages over polymerase chain reaction (PCR)–based techniques and commercially available PCR–based kits are expensive, this study was aimed to standardize simple in–house PCR–SSP technique.
- Accuracy of this test was further checked with standard PCR–SSOP (RLS) results.
- The frequency HLA–DRβ1*04 was significantly increased among RA patients when compared with normal controls.