Obese Mexican American Children Have Elevated MCP-1, TNF-alpha, Monocyte Concentration, and Dyslipidemia
Breslin WL et al. – Childhood obesity alters monocytes and circulating chemokines, putting children at a greater risk of developing obesity–related chronic diseases in adulthood. Further characterization of early immune alterations in childhood obesity may provide additional clinical insight into the assessment of obesity–related disease risk.Methods
- Blood samples were analyzed for total and subset monocyte concentration via flow cytometry.
- Serum monocyte chemoattractant protein-1 (MCP-1), fractalkine, interleukin-8, and tumor necrosis factor alpha (TNF-alpha) were measured by using a Milliplex MagPix assay.
- Serum cholesterol, high-density lipoproteins, triglycerides, and glucose were measured by using an enzymatic assay.
- Total monocyte concentration (P = .012), classic monocyte concentration (P = .045), MCP-1 (P = .015), and TNF-alpha (P = .002) were significantly greater in obese children compared with healthy weight children.
- Also, overweight and obese children had elevated triglycerides (P = .001) and reduced high-density lipoproteins (P = .033) compared with healthy weight children.