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Tracheal aspirate gene expression in preterm newborns and development of bronchopulmonary dysplasia
Pediatrics International, 04/26/2012
Hikino S et al. – High IL–10 expression in TAF cells at birth could predict the evolution of Bronchopulmonary dysplasia (BPD), but with less impact than oxygen requirement. Platelet–derived growth factor (PDGF) might play a different role in the inflammatory process of premature lungs.
Methods- Of 148 consecutive patients, 26 preterm infants (gestational age <34 weeks) were enrolled, who underwent assisted ventilation at birth for respiratory failure.
- Patients with congenital disorders were excluded. Half of these infants developed BPD.
- Interleukin (IL)-10, interferon (IFN)-gamma, transforming growth factor (TGF)-beta1, and platelet-derived growth factor (PDGF)-B mRNA of TAF cells were quantified on real-time polymerase chain reaction.
- IL-10 (P < 0.01) and IFN-gamma (P= 0.03) but not TGF-beta1 or PDGF-B mRNA levels at birth were higher in BPD than in non-BPD infants.
- IL-10 expression differentiated BPD with the highest sensitivity (92%) and specificity (77%).
- IL-10 levels correlated with TGF-beta1 (P= 0.03) and IFN-gamma (P= 0.01), but not with PDGF-B levels.
- When BPD infants were classified according to comorbidity (group 1, six patients who suffered respiratory distress syndrome [RDS] but not chorioamnionitis [CAM]; group 2, five patients who had CAM but not RDS), PDGF-B levels were higher in group 2 (P= 0.01).