Pediatrics Medical News about polyarticular juvenile idiopathic arthritis;differentiation,mineralization,apoptosis

Become a Member Today!
Register

Most Viewed Abstracts

1. Report Shows Shift in Starting Salaries for Physicians

2. Use of Antiemetic Agents in Acute Gastroenteritis

3. 2008 Exclusive Survey—Earnings: Good news for primary care income

4. Medicare pay-for-reporting effort draws fire from frustrated doctors

5. Debunking Myths in the US Healthcare System

Your Article Summary

See Latest Articles

(Click the title below to leave the MDLinx Network and go to the Journal's Website)

Serum from children with polyarticular juvenile idiopathic arthritis (pJIA) inhibits differentiation, mineralization and may increase apoptosis of human osteoblasts in vitro
Clinical Rheumatology, 08/12/08

Caparbo VF et al. - Serum from children with polyarticular juvenile idiopathic arthritis (pJIA) inhibits differentiation, mineralization and may increase apoptosis of human osteoblast cells (hOb) cultures, and inflammatory cytokines such as IL-6 might be a mechanism in this find.

Methods

Study to examine the effects of pJIA serum on proliferation, differentiation, mineralization, and apoptosis of hOb cells in culture
The hOb cells were tested for DNA synthesis, alkaline phosphatase (AP) activity, osteocalcin (OC) secretion, calcium levels, caspase 3 activity, and DNA fragmentation
None of the pts had used glucocorticoids or no other drug that can affect bone mineral metabolism for at least 1 mo before the study
Human inflammatory cytokine were measured in pJIA and CT sera

Results

Low levels of AP activity was observed in pJIA cultures vs CT cultures
There was also a decrease in OC secretion and calcium levels in pJIA hOb cultures
No difference in cell proliferation was observed
Osteoblasts cultured with JIA sera showed lower levels of DNA and increased fragmentation than osteoblasts cultured with CT sera
pJIA sera showed higher IL-6 values than CT
No difference was observed related to IL-8, IL-10, IL-1β, TNF-α, and IL-12p70 between pJIA and controls

<< Previous
An investigation on the mechanism of contralateral manifestations...

Next >>
2008 Exclusive Survey—Earnings: Good news for primary care income