Elevated levels of fibrinogen-derived endogenous citrullinated peptides in synovial fluid of rheumatoid arthritis patients Full Text
Arthritis Research & Therapy, 05/21/2012
Raijmakers R et al. – The increased presence of citrullinated peptides in Rheumatoid arthritis (RA) patients points towards a possible specific role of these peptides in the immune response at the basis of the recognition of citrullinated peptides and proteins by RA patient autoantibodies.Methods
- Endogenous peptides were isolated from the synovial fluid of RA patients and controls by filtration and solid phase extraction.
- The peptides were identified and quantified using high resolution liquid chromatography–mass spectrometry.
- The data reveal that the synovial fluid of RA patients contains soluble endogenous peptides derived from fibrinogen, containing significant amounts of citrulline residues and in some cases also phosphorylated serine.
- Several citrullinated peptides are found to be more abundantly present in the synovial fluid of RA patients compared to patients suffering from other inflammatory diseases affecting the joints.