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Increased levels of interleukin 33 in sera and synovial fluid from patients with active rheumatoid arthritis
Journal of Rheumatology, 11/18/09

Matsuyama Y et al. – IL-33 levels were elevated in sera and SF samples from patients with RA, and correlated with disease activity. IL-33 was produced mainly in inflamed joints; IL-33/ST2L signaling might play an important role in joint inflammation of human RA.

Methods

Sera obtained from 59 patients with RA, 10 patients with infectious diseases, and 42 healthy volunteers
SF samples obtained from 15 patients with RA and 13 with osteoarthritis
IL-33 levels measured using sandwich ELISA after removal of rheumatoid factor with protein A-Sepharose beads
FLS stimulated with IL-1ß and TNF, and treated with or without chemical damage
PBMC stimulated with anti-CD3/CD28 antibodies
Levels of IL-33 measured in culture supernatants and cell lysates by ELISA or immunoblotting

Results

Serum IL-33 levels significantly higher in RA patients, especially in high disease activity group compared to moderate or low activity group
IL-33 levels in SF elevated in all 15 RA patients measured
IL-33 levels higher in SF samples than in sera in 7 RA patients measured simultaneously
30-kDa IL-33 precursor detected in culture supernatants of damaged FLS but not detected in those of activated PBMC and non-damaged FLS