Ophthal Medical News

Become a Member Today!
Register

Most Viewed Abstracts

1. Report Shows Shift in Starting Salaries for Physicians

2. 2008 Exclusive Survey—Earnings: Good news for primary care income

3. Medicare pay-for-reporting effort draws fire from frustrated doctors

4. Debunking Myths in the US Healthcare System

5. Doctors and the DEA Free full text

Your Article Summary

See Latest Articles

(Click the title below to leave the MDLinx Network and go to the Journal's Website)

Are CD4+CD25-Foxp3+ cells in untreated new-onset lupus patients regulatory T cells?
Arthritis Research & Therapy, 10/14/09

Yang H et al. – The aim of this study was to explore the nature of abnormally increased CD4+CD25-Foxp3+ T cells in UNOL patients. CD4+CD25-Foxp3+ T cells in UNOL patients are different from regulatory T cells, both phenotypically and functionally. CD127 is not an appropriate surface marker for intracellular Foxp3 in CD4+CD25- T cells.

Methods

The expressions of surface (CD4, CD25, CD127, chemokine receptor 4 (CCR4), glucocorticoid induced tumor necrosis factor receptor (GITR) and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)) and intracellular molecules (Foxp3), as well as cytokine synthesis of peripheral blood mononuclear cells from twenty-two UNOL patients were analyzed by flow cytometry
Proliferative and suppressive capacities of different T cell subgroups from UNOL patients also assessed

Results

In UNOL patients, percentages of CD127low/- in CD25high, CD25low and CD25- subpopulations of CD4+Foxp3+ T cells were 93.79+/-3.48%, 93.66+/-2.31% and 91.98+/-2.14% respectively , whereas, the expressions of Foxp3 showed significant differences in CD25high (91.38+/-2.57%), CD25low (71.89+/-3.31%), and CD25- (9.02+/-2.21%) subpopulations of CD4+CD127low/- T cells
Expressions of surface CCR4, GITR and CTLA-4 on CD4+CD25-Foxp3+ T cells significantly less than CD4+CD25+Foxp3+ T cells
Moreover, different from CD4+CD25+Foxp3+ T cells, CD4+CD25-Foxp3+ T cells also synthesized interferon-gamma (IFN-gamma) interleukin (IL)-4IL-2 and IL-17(P<0.05), although less than CD4+CD25+Foxp3-T cells. The suppressive capacity most prominent in CD4+CD25highCD127low/-, followed by CD4+CD25lowCD127low/-
CD4+CD25-CD127- T cells showed least suppressive capacity which was similar to effector T cells