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See Latest ArticlesPreservation of the limbal stem cell phenotype by appropriate culture techniques
Investigative Ophthalmology and Visual Science, 09/16/09
Meyer–Blazejewska EA et al. – The proposed culture system supports enrichment and survival of limbal stem and progenitor cells during the entire cultivation process and may be essential for long–term restoration of the damaged ocular surface.
Ewa Anna Meyer-Blazejewska , 09/23/09
| The aim of this work was to provide an optimized culture technique to preferentially support isolation, ex vivo expansion and preservation of limbal stem cells prior to transplantation in patients with limbal stem cell deficiency. For this purpose we tested different variables including optimal limbal biopsy regions, cell isolation methods and culture conditions i.e. culture medium and supplement. We found that biopsies from the superior limbus, a gentle two-step enzymatic dissociation, clonal expansion of stem cells on a 3T3 feeder layer and finally subcultivation on a fibrin gel in low-calcium medium significantly increased the amount of stem cells prior to transplantation. Whether this culture technique can improve the therapeutic potential of limbal stem cell transplantation still remains to be evaluated. Nevertheless, this culture system might represent a starting point for establishing a true SC-based therapy for long-term ocular surface reconstruction. |
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