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Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function-spacer-lipid A and B constructs
Transfusion, 08/24/2011
Exclusive author commentary
Hult AK et al. – Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2μg/mL function–spacer–lipid KODE technology(FSL)–A or 78 to 10 μg/mL FSL–B transformation solutions. Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs.
Steve Henry (08/24/2011) comments:
This paper is part of a series of recent papers on KODE technology .KODE Technology is about a range of novel constructs that spontaneously and stably incorporate into membranes of cells and viruses or to label surfaces. These KODE constructs allow users to attach molecules such as saccharides including blood group-related determinants, sialic acids, hyaluronan, polysaccharides, fluorophores, biotin, radiolabels, and a range of peptides onto cells, viruses, liposomes and surfaces. These constructs have been successfully used in modifying embryos, spermatozoa, zebrafish, epithelial/endometrial cells, red blood cells, and virions to create quality controls systems and diagnostic panels, to modify cell adhesion/ interaction/ separation/ immobilization, and for in vitro and in vivo imaging of cells/virions. For more information search the internet for “kodecyte” or view the open access video "FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability" at http://www.jove.com/details.php?id=3289.