MicroRNA-182 downregulates metastasis suppressor 1 and contributes to metastasis of hepatocellular carcinoma Full Text
BMC Cancer, 06/13/2012
Wang J et al. – miR-182 could promote metastasis of HCC through the down-regulation of its target gene MTSS1. miR-182 and MTSS1 were potential prognostic markers and/or therapeutic targets in HCC.Methods
- miR-182 expression was tested in 86 cases of paired HCC and normal tissues by real-time PCR and the relationships between miR-182 expression and clinicopathological parameters were analyzed.
- The expression of MTSS1 was evaluated by immunohistochemistry and western blot in the above tissues and its correlation with miR-182 expression was analyzed.
- Moreover, western blot and invasion assay were performed after transfection of pre-miR-182 or anti-miR-182 in HCC cell lines.
- In addition, luciferase assay and rescue experiment were performed in HCC cell lines.
- Compared with normal tissue, miR-182 was up-expression and MTSS1 was down-expression in HCC tissues.
- Moreover, the over-expression of miR-182 was correlated with intrahepatic metastasis (p=0.034) and poor prognosis (p=0.039) of HCC patients.
- There was a negative correlation between miR-182 and MTSS1 expression both in HCC tissues (r =-0.688, p<0.01) and HCC cell lines (r =-0.931, p=0.021).
- Furthermore, the up-regulation of miR-182 resulted in the down-expression of MTSS1 and increased invasive potential of HUH-1, and reverse results were also confirmed when the expression of miR-182 was inhibited.
- In addition, the results of luciferase assay demonstrated the targeted regulation of miR-182 on MTSS1.