Tumor-associated macrophages in pediatric classical Hodgkin lymphoma: association with Epstein-Barr virus, lymphocyte subsets and prognostic impact
Clinical Cancer Research, 06/05/2012
Barros MHM et al. – Our results suggest that macrophage composition in pediatric cHL is distinct from adults. Functional status of macrophages and their value as prognostic indicators in pediatric cHL may depend on EBV status.Methods
- We analyzed the number of macrophages and dendritic cells (DCs) in the tumor microenvironment of pediatric cHL by immunohistochemistry (IHC).
- Results were analyzed in context of age, histological characteristics, EBV-status, clinical follow-up and our previous study of T-cell populations in these cases.
- 100 cHL cases were studied, including 69% nodular sclerosis (NS) and 23% mixed cellularity (MC) cases.
- 44.8% of cases were EBV-positive.
- Patients ?10 years displayed more CD14+ cells (P= 0.025).
- In comparison to NS, MC was characterized by higher numbers of CD14+, (P= 0.003) and CD163+ cells (P= 0.027).
- EBV+ cases exhibited higher numbers of CD14+ (P< 0.0005), CD68+ (P= 0.005) and CD163+ cells (P= 0.02).
- CD68-positive cells did not display an effect on outcome. Worse overall survival was observed in cases with CD163/CD8 ratio >/= 2 (P= 0.007).
- High numbers of CD163+ cells were associated with worse progression-free survival (PFS) (P= 0.015).
- Furthermore, high numbers of CD163+ and Granzyme B+ cells were associated with worse PFS in EBV-negative (P= 0.005) but not in EBV-positive cases.