mdlinx mdlinx
Latest (99) Full Text Articles (6313) Focus on CRC Article Summary

Peripheral T-lymphocytes express WNT7A and its restoration in leukemia-derived lymphoblasts inhibits cell proliferation Full Text
BMC Cancer,

Ochoa-Hernandez AB et al. – To the knowledge of the authors, this is the first report evidencing quantitatively decreased WNT7A levels in leukemia-derived cells and that WNT7A restoration in T-lymphocytes inhibits cell proliferation. These results also support the possible function of WNT7A as a tumor suppressor gene as well as a therapeutic tool.

Methods

  • Analyzed peripheral blood mononuclear cells, sorted CD3 and CD19 cells, 4 leukemia-derived cell lines, and blood samples from 14 patients with Acute lymphoblastic leukemia (ALL), and 19 clinically healthy subjects
  • Reverse transcription followed by quantitative Real-time Polymerase chain reaction (qRT-PCR) analysis were performed to determine relative WNT7A expression
  • Restoration of WNT7a was done employing a lentiviral system and by using a recombinant human protein
  • Cell proliferation was measured by addition of WST-1 to cell cultures

Results
  • WNT7a is mainly produced by CD3 T-lymphocytes, its expression decreases upon activation, and it is severely reduced in leukemia-derived cell lines, as well as in the blood samples of patients with ALL when compared with healthy controls (p [less than or equal to]0.001)
  • By restoring WNT7A expression in leukemia-derived cells, we were able to demonstrate that WNT7a inhibits cell growth
  • Similar effect was observed when a recombinant human WNT7a protein was used
  • Restoration of WNT7A expression in Jurkat cells did not activate the canonical Wnt/beta-catenin pathway

Please login or register to follow this author.
Are you sure you want to Unfollow this Author?
► Click here to access Full Text, PubMed, Publisher and related articles...
<< Previous Article | Next Article >>

Your Unread Messages in Oncology

See All >> Messages include industry-sponsored communications and special communications from MDLinx

Most Popular Oncology Articles

Last month's top read Top Articles of 2013

1 Coffee intake and gastric cancer risk Cancer Epidemiology, Biomarkers & Prevention, March 20, 2014    Clinical Article

2 Rituximab versus a watch-and-wait approach in patients with advanced-stage, asymptomatic, non-bulky follicular lymphoma: an open-label randomised phase 3 trial The Lancet Oncology, March 7, 2014    Clinical Article

3 Carboplatin plus paclitaxel once a week versus every 3 weeks in patients with advanced ovarian cancer (MITO-7): a randomised, multicentre, open-label, phase 3 trial The Lancet Oncology, March 11, 2014    Clinical Article

4 Effect of radiotherapy after mastectomy and axillary surgery on 10-year recurrence and 20-year breast cancer mortality: meta-analysis of individual patient data for 8135 women in 22 randomised trials The Lancet, March 25, 2014    Evidence Based Medicine    Clinical Article

5 Effects of adjuvant exemestane versus anastrozole on bone mineral density for women with early breast cancer (MA.27B): A companion analysis of a randomised controlled trial The Lancet Oncology, March 14, 2014    Clinical Article

6 Chemoradiation therapy versus chemotherapy alone for gastric cancer after R0 surgical resection: A meta-analysis of randomized trials Oncology, February 4, 2014    Evidence Based Medicine    Clinical Article

7 Risk of developing myelodysplastic syndromes in prostate cancer patients definitively treated with radiation Journal of the National Cancer Institute, March 4, 2014    Clinical Article

8 Screening for prostate cancer with the prostate-specific antigen test: A review of current evidence JAMA, March 21, 2014    Evidence Based Medicine    Clinical Article

9 Dietary carrot consumption and the risk of prostate cancer European Journal of Nutrition, February 17, 2014    Clinical Article

10 Second primary malignancies with lenalidomide therapy for newly diagnosed myeloma: A meta-analysis of individual patient data The Lancet Oncology, March 7, 2014    Clinical Article

11 A comparison of carboplatin and paclitaxel with cisplatinum and 5-fluorouracil in definitive chemoradiation in esophageal cancer patients Annals of Oncology, February 18, 2014    Clinical Article

12 Radical prostatectomy or watchful waiting in early prostate cancer New England Journal of Medicine, March 10, 2014    Evidence Based Medicine    Clinical Article

13 Coffee consumption and prostate cancer risk: An updated meta-analysis Cancer Causes and Control, March 4, 2014    Evidence Based Medicine    Clinical Article

14 Statins and aspirin for chemoprevention in Barrett's esophagus: Results of a cost-effectiveness analysis Cancer Prevention Research, February 25, 2014    Clinical Article

15 Neoadjuvant and adjuvant trastuzumab in patients with HER2-positive locally advanced breast cancer (NOAH): Follow-up of a randomised controlled superiority trial with a parallel HER2-negative cohort The Lancet Oncology, March 24, 2014    Clinical Article

16 Survival, durable tumor remission, and long-term safety in patients with advanced melanoma receiving nivolumab Journal of Clinical Oncology, March 19, 2014    Clinical Article

17 Tea consumption and leukemia risk: A meta-analysis Tumor Biology, February 13, 2014    Evidence Based Medicine    Clinical Article

18 Vemurafenib in patients with BRAFV600 mutated metastatic melanoma: an open-label, multicentre, safety study The Lancet Oncology, March 3, 2014    Clinical Article

19 Yoga’s impact on inflammation, mood, and fatigue in breast cancer survivors: a randomized controlled trial Journal of Clinical Oncology, February 3, 2014    Clinical Article

20 Tumor infiltrating lymphocytes is prognostic and predictive for trastuzumab benefit in early breast cancer: results from the FinHER trial Annals of Oncology, March 12, 2014    Clinical Article

Indexed Journals in Oncology: Journal of Clinical Oncology, Cancer Research, Annals Oncologymore

Other Topics in Oncology

Register now to view all the MDLinx contents (FREE)!

  • Stay current on the latest literature, research and clinical news
  • Get special communications and offers from MDLinx and our sponsors
  • Receive invitations to paid market research
View Samples and Register

Stay current - Media Tool

Newsletter
RSS
Follow
Like

Receive the latest mecial news
updates for free via email

Sign up!

Subscribe to our free RSS feeds:

Get the latest news in your specialty automatically added to your newsreader or your personal My Yahoo!, Google, My MSN or My AOL page. Learn More

Close