Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells

Cancer Immunology, Immunotherapy, 11/12/2009

The serum task force results demonstrate compellingly that serum–free media perform as well as qualified medium/serum combinations, independent of the applied SOP. Recovery and viability of cells are largely unaffected by serum–free conditions even after overnight resting. Furthermore, one serum–free medium was identified that appears to enhance antigen–specific IFNgamma–secretion.

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