CINtec plus dual immunostain: A triage tool for cervical pap smears with atypical squamous cells of undetermined significance and low grade squamous intraepithelial lesion
Diagnostic Cytopathology, 07/27/2012
Loghavi S et al. – Cervical intraepithelial neoplasia (CIN)tec plus is a helpful adjunct in identifying underlying (U) or subsequent (S) high grade cervical intraepithelial neoplasia (HG CIN) when applied to SurePath Pap smears with ASC or LSIL.Methods
- The authors evaluated the role of the CINtec PLUS p16/Ki-67 dual immunostain as a marker for underlying (U) or subsequent (S) HG CIN.
- One hundred and eighty eight cervical SurePath Pap smears with histological and/or cytological follow-up were retrieved from the departmental files.
- The Pap stained slides were destained and then immunostained utilizing the CINtec PLUS dual staining reagent kit.
- Results of the dual stain were correlated with follow-up diagnoses.
- Sensitivity, specificity, and positive and negative predictive values of CINtec PLUS for U or S HG CIN were compared with those of HR HPV testing and with p16 and Ki-67 immunostaining alone.
- The sensitivity of CINtec PLUS for U or S HG CIN was 91% in the ASC group and 100% in the LSIL group, while the corresponding specificities were 61 and 43%, respectively.
- The sensitivity and specificity of CINtec PLUS for U or S HG CIN in both groups combined were 97 and 53%, respectively.
- CINtec PLUS was more specific than HR HPV testing and Ki-67 and p16 immunostains alone in detecting an U or S HG CIN.