Same-day prenatal diagnosis of common chromosomal aneuploidies using microfluidics-fluorescence in situ hybridization
Prenatal Diagnosis,

Ho SSY et al. – Results were concordant with their karyotypes and ready to be released within 3?h of sample receipt. Microfluidic FISH, using 20–fold less than the recommended amount of probe, is a cost–effective method to diagnose common fetal aneuploidies within the same day of fetal sampling.

  • Rapid molecular prenatal diagnostic methods, such as fluorescence in situ hybridization (FISH), quantitative fluorescence–PCR, and multiplex ligation–dependent probe amplification, can detect common fetal aneuploidies within 24 to 48?h.
  • However, specific diagnosis or aneuploidy exclusion should be ideally available within the same day as fetal sampling to alleviate parental anxiety.
  • Microfluidic technologies integrate different steps into a microchip, saving time and costs.
  • Authors have developed a cost–effective, same–day prenatal diagnostic FISH assay using microfluidics.
  • Amniotic fluids (1–4?mL from 40 pregnant women at 15–22 weeks of gestation) were fixed with Carnoy's before loading into the microchannels of a microfluidic FISH–integrated nanostructured device.
  • The glass slides were coated with nanostructured titanium dioxide to facilitate cell adhesion.
  • Pretreatment and hybridization were performed within the microchannels. Fifty nuclei were counted by two independent analysts, and all results were validated with their respective karyotypes.
  • Fifty nuclei were counted by two independent analysts, and all results were validated with their respective karyotypes.
  • Of the 40 samples, we found three cases of fetal aneuploidies (trisomies 13, 18, and 21), whereas the remaining 37 cases were normal.

Please login or register to follow this author.
Are you sure you want to Unfollow this Author?
► Click here to access PubMed, Publisher and related articles...
<< Previous Article | Next Article >>

Your Unread Messages in Obstetrics & Gynecology

See All >> Messages include industry-sponsored communications and special communications from MDLinx

Most Popular Obstetrics & Gynecology Articles

1 Egg consumption is associated with increased risk of ovarian cancer: Evidence from a meta-analysis of observational studies Clinical Nutrition, August 5, 2014    Evidence Based Medicine

2 Menopausal hormone therapy use in 17 European countries during the last decade Maturitas, August 14, 2014    Clinical Article

3 Soda consumption and risk of hip fractures in postmenopausal women in the Nurses’ Health Study American Journal of Clinical Nutrition, August 18, 2014    Clinical Article

4 Intake of dietary flavonoids and risk of epithelial ovarian cancer American Journal of Clinical Nutrition, August 26, 2014    Clinical Article

5 A comparison of multiphasic oral contraceptives containing norgestimate or desogestrel in acne treatment: A randomized trial Contraception, August 12, 2014    Clinical Article

6 Metformin in reproductive health, pregnancy and gynaecological cancer: Established and emerging indications Human Reproduction Update, July 24, 2014    Review Article

7 Dietary intake and weight gain among adolescents on depot medroxyprogesterone acetate Journal of Pediatric and Adolescent Gynecology , August 7, 2014    Clinical Article

8 Age of menarche and time to pregnancy Human Reproduction, August 27, 2014    Clinical Article

9 A revised birth weight reference for the United States Obstetrics and Gynecology, July 14, 2014    Clinical Article

10 Maternal caffeine consumption and small for gestational age births: Results from a population-based case-control study Maternal and Child Health Journal , July 31, 2014    Clinical Article

11 Effects of omega-3 fatty acid supplementation on insulin metabolism and lipid profiles in gestational diabetes: Randomized, double-blind, placebo-controlled trial Clinical Nutrition, August 6, 2014    Clinical Article

12 Effect of oral magnesium supplementation on physical performance in healthy elderly women involved in a weekly exercise program: A randomized controlled trial American Journal of Clinical Nutrition, July 17, 2014    Clinical Article

13 Dietary intake and weight gain among adolescents on depot medroxyprogesterone acetate Journal of Pediatric and Adolescent Gynecology , August 11, 2014    Clinical Article

14 Perineal outcome and the risk of pelvic floor dysfunction: A cohort study of primiparous women Australian and New Zealand Journal of Obstetrics and Gynaecology, August 28, 2014    Clinical Article

15 Efficacy of combination therapy of oral zinc sulfate with imiquimod, podophyllin or cryotherapy in the treatment of vulvar warts Journal of Obstetrics and Gynaecology Research, August 22, 2014    Clinical Article

16 The role of dietary protein and vitamin D in maintaining musculoskeletal health in postmenopausal women: A consensus statement from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) Maturitas, August 1, 2014    Clinical Article

17 Vitamin D deficiency and pregnancy rates in women undergoing single embryo, blastocyst stage, transfer (SET) for IVF/ICSI Human Reproduction, August 25, 2014    Clinical Article

18 The risk of prelabor and intrapartum cesarean delivery among overweight and obese women: Possible preventive actions American Journal of Obstetrics and Gynecology, August 20, 2014    Clinical Article

19 Pilot study of integral yoga for menopausal hot flashes Menopause, August 8, 2014    Clinical Article

20 The effect of chromium supplementation on polycystic ovary syndrome in adolescents Journal of Pediatric and Adolescent Gynecology , May 30, 2014    Clinical Article

Indexed Journals in Obstetrics & Gynecology: Obstetrics and Gynecology, American Journal of Obstetrics and Gynecologymore

Register now to view all the MDLinx contents (FREE)!

  • Stay current on the latest literature, research and clinical news
  • Get special communications and offers from MDLinx and our sponsors
  • Receive invitations to paid market research
View Samples and Register

Connect with us, stay current.

Receive the latest mecial news
updates for free via email

Sign up!

Subscribe to our free RSS feeds:

Get the latest news in your specialty automatically added to your newsreader or your personal My Yahoo!, Google, My MSN or My AOL page. Learn More

Close