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Correlation of GREB1 mRNA with protein expression in breast cancer: validation of a novel GREB1 monoclonal antibody
Breast Cancer Research and Treatment, 11/04/09

Hnatyszyn HJ et al. – These data indicate that GREB1 mRNA expression correlates well with protein expression. The novel monoclonal GREB1ab is specific for GREB1 protein. This antibody will serve as a tool for investigations focused on the expression, distribution, and function of GREB1 in normal breast and breast cancer tissues.

Methods

A hybridoma expressing a murine monoclonal antibody targeting a 119 amino acid peptide specific to human GREB1 was generated
GREB1ab detects a 216 kDa protein corresponding to GREB1 in estrogen receptor alpha (ER?+) breast cancer cells as well as ER?? breast cancer cells transduced with a GREB1 expression vector

Results

ER?+ cell lines were observed to express GREB1 while ER?? cell lines did not express detectable levels of the protein
Using breast cancer tissue whole sections, IHC with the GREB1ab identified protein expression in ER?+ breast cancer tissue as well as normal breast tissue, with little GREB1 expression in ER?? breast cancer tissue