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Li Y et al. – Several factors have been suggested to explain the increase in monozygotic multiple pregnancies after assisted reproductive technique (ART): advanced maternal age, superovulation, and manipulation of the zona pellucida (ZP) including ICSI and assisted hatching, prolonged culture, and in vitro culture condition. All patients should be informed that monozygotic multiple pregnancies occur at a significantly higher rate after ART procedures and could be an important complication after IVF–ICSI treatment.


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