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Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm
Fertility and Sterility, 09/14/09
Santiso R et al. – Deoxyribonucleic acid fragmentation and oxidative DNA damage were simultaneously determined in the same sperm cell, incubating with an 8–oxoguanine DNA probe on human spermatozoa processed by the sperm chromatin dispersion test. The assay was validated by incubation with agents that induce DNA fragmentation with or without oxidative base damage.
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