Neurology Medical News about capillary, immunofluorescence, Ki-67

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The expression of vascular endothelial growth factor in skeletal muscle of patients with sleep disorders
Muscle & Nerve, 07/24/09

Wåhlin-Larsson B et al. – Findings show the occurrence of proliferation of endothelial cells in skeletal muscle in restless legs syndrome (RLS) and obstructive sleep apnea syndrome (OSAS), supporting an upregulation of vascular endothelial growth factor (VEGF) located in capillaries, probably due to local hypoxia.

Methods

Study of the occurrence and localization of VEGF and capillary proliferation in skeletal muscle of 12 RLS and 12 OSAS pts and 11 controls
Double-immunofluorescence staining for capillaries (CD31) and VEGF and proliferating cells (Ki-67) for tibialis anterior biopsies

Results

Significantly higher percentage of capillaries expressing VEGF (CD31,VEGF+) in OSAS and RLS pts vs controls
Significantly higher percentage of proliferating capillaries (CD31,Ki-67+) for OSAS pts vs controls

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