Specific detection of enteroaggregative hemorrhagic Escherichia coli O104:H4 strains using the CRISPR locus as target for a diagnostic real-time PCR.
Journal of Clinical Microbiology, 08/16/2012
Delannoy S et al. – The 35 E. coli O104 strains expressing other H–types than H4 as well as eight E. coli strains carrying a K9 capsular antigen tested all negative for the CRISPRO104:H4 locus. Only twelve (0.94%) of the 1273 non–O104:H4 E. coli strains (serotypes Ont:H2, O43:H2, O141:H2, and O174:H2) reacted positive in the CRISPRO104:H4 PCR (99.06% specificity).