Expansions of CD4+CD28- and CD8+CD28- T Cells in Granulomatosis with Polyangiitis and Microscopic Polyangiitis Are Associated with Cytomegalovirus Infection But Not with Disease Activity

The Journal of Rheumatology, 08/09/2012

Eriksson P et al. – CD28- and CD57+ T cells were associated with latent cytomegalovirus (CMV) infection and not with a diagnosis of granulomatosis with polyangiitis or microscopic polyangiitis. Vasculitis assessment should include CMV status.

Methods

  • Blood from 16 patients in remission (12 GPA, 4 MPA), 18 patients with active vasculitis (12 GPA, 6 MPA), and 20 healthy controls was examined by flow cytometry for expression of CD4, CD8, CD56, CD57, and CD28 on T cells.
  • The influence of age, CMV status, presence of disease, and disease activity on T cell subpopulations was tested with multiple regression analyses.

Results

  • In active vasculitis, the total numbers and proportion of lymphocytes were decreased.
  • Total numbers of CD4+, CD8+, CD4+CD28-, CD8+CD28-, CD4+CD57+, and CD8+CD57+ T subpopulations were decreased to the same extent, implying unchanged proportions.
  • Multivariate analyses showed no associations between vasculitis and CD28- or CD57+ T subpopulations, whereas immunoglobulin G antibodies to CMV were associated with expanded proportions of CD28- and CD57+ T cells, in both the CD4+ and the CD8+ compartments.

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