Terminal Restriction Fragment Length Polymorphism Analysis of the Gut Microbiota Profiles of Pediatric Patients with Inflammatory Bowel Disease
Aomatsu T et al. – The gut microbiota profile of pediatric Crohn’s disease (CD) patients was different from that of healthy children.Methods
- Terminal restriction fragment length polymorphism analysis was performed in 10 fecal samples from Crohn’s disease (CD), 14 samples from ulcerative colitis (UC) and 27 samples from healthy individuals.
- The bacterial diversity was evaluated by the Shannon diversity index.
- In CD patients, a setting of similarity generated three major clusters.
- The majority of CD patients were classified into CD clusters I and II (9 out of 10), but the majority of healthy individuals (21 of 27) were classified into CD cluster III.
- In UC patients, a setting of similarity also generated three major UC clusters, but each cluster was not characteristic for UC patients or healthy individuals.
- The changes in simulated bacterial composition indicated that the class Clostridia, including the genus Faecalibacterium, was significantly decreased in CD patients as compared to UC patients and/or healthy individuals.
- The genus Bacteroides was also decreased as compared to healthy individuals.
- The bacterial diversity measured by the Shannon diversity index was significantly reduced in CD patients as compared to healthy individuals.