Herpes Simplex Virus Glycoproteins gH/gL and gB Bind Toll-Like Receptor 2, and Soluble gH/gL Is Sufficient To Activate NF-κB
Journal of Virology, 06/04/2012
Leoni V et al. – A soluble form of gB, truncated upstream of the transmembrane sequence (gB730t–st), was produced in human cells and purified by means of a Strep tag. gH/gL and gB were each able to physically interact with TLR2 in coimmunoprecipitation assays, one independently of the other, yet gHt–st/gL, but not gB730t–st, elicited an NF–κB response. Thus, whereas both HSV gH/gL and gB are ligands to TLR2, only gH/gL is sufficient to initiate a signaling cascade which leads to NF–κB activation.