Identification and validation of reference genes for expression studies in human keratinocyte cell lines treated with and without interferon- γ a method for qRT-PCR reference gene determination
Experimental Dermatology, 06/08/2012
Riemer AB et al. – The authors conclude that the geometric means of expression values of these four genes represents a robust normalization factor for quantitative reverse–transcription real–time polymerase–chain–reaction (qRT–PCR) analyses in interferon–γ–dependent gene expression studies in keratinocytes. The methodology and results herein may help other researchers by facilitating their choice of reference genes.