Structural alterations of the mucosa stroma in the Barretts esophagus metaplasia-dysplasia-adenocarcinoma sequence
Journal of Gastroenterology and Hepatology, 06/08/2012
Bobryshev YV et al. – The study demonstrates distinctive alterations of the mucosa stroma extracellular matrix in the metaplasia–dysplasia–adenocarcinoma sequence. The findings suggest that the redistribution of collagen fibers and increases in numbers of matrix microvesicles may play roles in the formation of specialized intestinal metaplasia and the development of adenocarcinoma.Methods
- A total of 41 esophageal tissue specimens (15 esophageal adenocarcinoma, 10 Barrett's esophagus intestinal metaplasia, 7 dysplasia and 9 normal esophagus) were studied.
- The present study utilized transmission electron microscopy and computerized quantitative electron-microscopic analysis in order to investigate the characteristics of the extracellular matrix of the mucosa.
- The study revealed that marked structural alterations of the mucosa stroma, relating to changes in the distribution and appearance of collagen fibers as well as to changes in numbers of matrix microvesicles, occur in Barrett's esophagus and esophageal adenocarcinoma.
- It was found that there were 3.1 times more microvesicles in the stroma in Barrett's esophagus than in the stroma of the normal esophagus (p<0.0001) and that there were 5.8 times more microvesicles in esophageal adenocarcinoma than in the normal esophagus (p<0.0001).
- There were 1.9 times more microvesicles in esophageal adenocarcinoma than in Barrett's esophagus (p=0.0043).