Detection and genotyping of human papillomaviruses and their role in the development of ovarian carcinomas
Archives of Gynecology and Obstetrics, 05/30/2012
Malisic E et al. – The results indicate that HPV infection may play a limited role in ovarian carcinogenesis.Methods
- Fifty–four ovarian carcinoma patients were included in the study.
- DNA was isolated by salting out method from tumor tissue obtained after surgical treatment.
- Presence of HPV infection was detected through polymerase chain reaction amplification of a 150–bp fragment of L1 viral gene by GP5+/GP6+ primers. HPV genotyping was performed by DNA sequencing.
- HPV DNA was present in 4/54 (7.4 %) ovarian carcinomas.
- All HPV–positive tumors contained high–risk HPV16 type. HPV infection was more common in advanced opposite to localized disease.
- The median age of diagnosis of disease varied from 57 years for patients with HPV infection to 59 years for patients without HPV infection.