Coverage evaluation of universal bacterial primers using the metagenomic datasets Full Text
BMC Microbiology, 05/14/2012
Mao DP et al. – The study revealed the potential bias introduced by the use of universal bacterial primers in the assessment of microbial communities. With the development of high–throughput, next–generation sequencing techniques, it will become feasible to sequence more of the hypervariable regions of the bacterial 16S rRNA gene. This, in turn, will lead to the more frequent use of the primers discussed here.