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Identification of RGS1 as a candidate biomarker for undifferentiated spondylarthritis by genome-wide expression profiling and real-time polymerase chain reaction
Arthritis & Rheumatism, 11/09/09

Gu J et al. – Findings indicate that uSpA PBMCs carry strikingly more highly expressed genes compared with PBMCs from AS patients or healthy subjects, and that TNF- and IL-17-inducible RGS1 is a potential biomarker for uSpA, and to a lesser extent for AS, with inflammatory low back pain.

Methods

Peripheral blood mononuclear cells (PBMCs) from patients with AS, patients with uSpA, and healthy subjects screened using genome-wide microarrays
Followed by validation by real-time polymerase chain reaction (PCR)

Results

Microarray profiling and real-time PCR assays showed only minor differences between AS patients and healthy subjects
20 genes strikingly more highly expressed in uSpA patients
Regulator of G protein signaling 1 (RGS1) identified as most useful biomarker for distinguishing uSpA patients, and to lesser extent AS patients, from control subjects
Findings verified in independent cohort that also included patients with RA and patients with mechanical low back pain
To evaluate possible derivation of RGS1, we cultured a monocyte-derived cell line with panel of cytokines and chemokines