Morito T et al. - Synovial fluid-derived mesenchymal stem cells (SF-MSCs), similar to synovium mesenchymal stem cells (MSCs), increased in number after intra-articular (IA) ligament injury and surgery without marked alteration of the properties. Methods
Aim was to study the behaviour and properties of MSCs derived from SF
Human SFs from 19 knee joints with anterior cruciate ligament injury around the time of reconstruction surgery, and 3 healthy volunteers
SF was plated, cultured and examined for colony-forming number, in vitro differentiation, surface epitopes and gene profiles
Also, rabbit synovium-MSCs were injected into the knee joint in a rabbit partial anterior cruciate ligament defect model, and the injected cells were traced
Results
SF-MSCs from IA ligament injury pts were 100 times more in number vs healthy volunteers
Total colony number was positively correlated with post-injury period
No differences among the cells derived from SF around the time of the surgery in relation to surface epitopes and differentiation potentials
SF-MSCs were more similar to synovium MSCs than bone marrow MSCs
MSCs injected into the knee in which IA ligament was partially defective were observed more on the defected area vs intact area of the ligament at 24 h
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